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4/24/2023
On the General Microbiology Test Request Form (OHA0060), in the “Mycobacteria” section, check the “AFBPCR: AFB Culture Identification and PCR; isolate” box. Enter Date Culture Positive next to test order.
All clients - Mycobacteriology labs
Wednesdays and Fridays on regular business days
Within 6 working days after receipt of specimen
Actively growing isolated acid fast organism, on solid or broth media
Detailed instructions for collection, packaging, and submission of specimens (pdf)
AFB Growth on solid media, or 1.0ml broth culture of AFB with turbidity > 1 Mcfarland turbidity
Solid media: visible growth.
Seal tube, place in biohazard transport bag, place in a secondary transport container, and incubate at 37°C or store at room temperature (20-25°C) pending transport.
Package specimens to maintain room temperatures during transport.
Isolates not confirmed as acid fast organisms.
Specimens are subject to the requirements of the OSPHL Specimen Submission Policy (pdf).
Isolates identified as MTBC are retained for 6 months after receipt at the laboratory. Isolates identified as MAC, non-speciated NTM, or Indeterminate, are retained for 3 months after receipt at the laboratory.
For additional information or assistance, please contact the OSPHL at 503-693-4100.
Multiplex real-time semi-quantitative PCR
Comprehensive changes to detailed instructions attachment, review as new; Edits to How to Order, Handling, Rejection Criteria, Performance Specifications
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