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Lab Test Details

Viral Gastroenteritis Panel, Real-Time RT-PCR

Updated

5/23/2023

Search TermsAstrovirus; Calicivirus; Norovirus; Sapovirus; Rotavirus; Gastrointestinal Viruses; Norwalk
How to Order
On the Virology/Immunology Test Request Form (OHA0042), in the “Molecular” section, check the “MOL NOV: Norovirus RT-PCR” box.
Availability
With approval from the Acute and Communicable Disease Program or Local Public Health Authorities
Performed

As needed

Time to Reporting
Within 7 working days after receipt of specimen
CPT Code
87798 x 2 if Norovirus positive
If Norovirus is negative, 87798 repeated for each additional gastrointestinal pathogen test performed


Specimen Collection Details

Collection
Human stool in a clean, screw-top plastic container or stool in Cary Blair enteric pathogen transport media.
 
Vomit in a screw top plastic container is acceptable, but not preferred.
Optimal Volume
Raw Stool - 2 mL or a "walnut-sized” amount
 
Solid or Liquid Stool; Vomit - 5 gm or 5 mL
Minimum Volume
Raw Stool - 1 mL or a “pea-sized” amount
 
Solid Stool - 2 gm 

Liquid Stool; Vomit - 5 mL
Handling

Store specimens at refrigerated temperatures (2-8°C) pending transport. Transport as soon as possible after specimen collection.

Transport

Package specimens with fully frozen ice packs to maintain refrigerated temperatures during transport.

Specimens should be transported to be received at the OSPHL within 5 days of specimen collection.

Rejection Criteria
Specimens received more than 10 calendar days after specimen collection.
 
Specimens are subject to the requirements of the OSPHL Specimen Submission Policy and Criteria, available at: http://bit.ly/SpecimenCriteria.
Comments
Original material submitted for testing is retained for 7 calendar days after receipt at the laboratory.

Norovirus is the major cause of viral gastroenteritis. Other gastrointestinal viruses (astrovirus, sapovirus, and rotavirus) will only be tested upon approval from state or local public health authorities.

If bacterial pathogens are suspected, follow the instructions for Enteric Pathogen Culture.

Method Details

Methodology

Real-Time Reverse-Transcription Polymerase Chain Reaction

Performance Specifications
Method Performance Specifications determined by the Centers for Disease Control and Prevention (CDC). Please contact the CDC for specifications if needed.

Change Log

5/23/2023
Edit How to Order, CPT Code, Comments
3/15/2021

Edit Test Name and Performance Specifications