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Lab Test Details


Carbapenem-Resistant Organisms (CRO) Testing

Updated

3/13/2024

Search TermsCarbapenem-Resistant Enterobacterales (CRE) PCR; KPC and NDM; CRE Screen; Carba NP; IMP; VIM; OXA-48 in Enterobacterales; Formerly Enterobacteriaceae; Pseudomonas aeruginosa (CRPA); Acinetobacter species (CRA)
How to Order On the General Microbiology Test Request Form (OHA0060), in the “Miscellaneous Testing” section, check the “CRE” or "CRPA" box. Include the Organism name and attach your laboratory's susceptibility testing results.
Availability

All clients

PerformedTuesdays, Thursdays, and Fridays on regular business days
Time to Reporting
Within 5 working days after receipt of specimen
Public Health Data
De-identified data from antimicrobial surveillance testing are shared with the Centers for Disease Control and Prevention as part of the Antimicrobial Resistance Laboratory Network. 
CPT Code

87798 x 2, 87185


Specimen Collection Details

Collection Actively growing isolates of E. coli, Klebsiella spp., Enterobacter spp., Pseudomonas aeruginosa, Acinetobacter spp., or other Enterobacterales species.

Submit isolates on an agar slant or non-selective plate media.
Optimal Volume

N/A

Minimum Volume

N/A

HandlingSeal plate or tube, place in biohazard transport bag.

Store specimens at ambient temperatures (20-25°C) pending transport.

Do not freeze, avoid extreme heat.
Transport

Package specimens to maintain ambient temperatures during transport.

Rejection CriteriaMixed culture

Specimens are subject to the requirements of the OSPHL Specimen Submission Policy and Criteria, available at: http://bit.ly/SpecimenCriteria.
Comments Original material submitted for testing is retained for 5 calendar days after receipt at the laboratory.

For additional information or assistance, please contact the OSPHL at 503-693-4100.

Method Details

Methodology

Carba NP Test, Multiplex Real-Time PCR, Susceptibility testing by broth microdilution 

Performance Specifications
Carba NP:
  • Percent accuracy - 92.18%
IMP/VIM PCR: 
  • Percent accuracy - 100%
  • Limit of detection (LOD)
    • IMP - 1000 genome copies/uL
    • VIM - 10 genome copies/uL
  • Specificity - 100%
OXA-48 PCR:
  • Percent accuracy - 100%
  • Limit of detection - 10 genome copies/uL
  • Specificity - 100%
KPC/NDM PCR:
  • Percent accuracy - 100%
  • Analytical Sensitivity or Limit of Detection (LOD)
    • KPC - 100 genome copies
    • NDM - 1000 genome copies
  • Specificity - 100%
Broth microdilution:
  • Percent accuracy - >90%
  • Precision/Reproducibility - >95%

Additional method performance specifications may be available. Contact the OSPHL if needed.


Change Log

3/13/2024
New test menu page, replaces CRE test menu page